リンゴ台木'マルバカイドウ長野No.1'におけるAgrobacterium rhizogenesにより形成された毛状根からの植物体再分化
書誌事項
- タイトル別名
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- Plant Regeneration from Hairy Roots of Apple Rootstock, Malus prunifolia Borkh. var. ringo Asami, Strain Nagano No.1, Transformed by Agrobacterium rhizogenes
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Stems of Mains prunifolia Borkh. var. ringo Asami strain Nagano No.l were infected with Agrobacterium rhizogenes MAFF-02-10265, MAFF-02-10266, MAFF-03-01724 and MAFF-03-01725 by using a needle. The frequency of hairy root formation was 2.0% with both MAFF-02-10265 and MAFF-02-10266. Transgenic plants were obtained from hairy roots that were induced by A. rhizogenes MAFF-02-10265. Adventitious shoots were formed from the hairy roots on Murashige and Skoog medium, supplemented with 5.0 mg·liter-1 6-benzyl-aminopurine (BAP), 0.1 mg·liter-1 indole-3-butyric acid (IBA) and 0.1 mg·liter-1 gibberellic acid (GA3). These shoots developed abundant roots in the culture medium. The transgenic nature of the regenerated plants was confirmed by opine and PCR analysis. A transgenic plant, acclimatized in the greenhouse, showed prolific branching, miniaturized leaves, and shortened internodes.
収録刊行物
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- 園芸学会雑誌
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園芸学会雑誌 73 (6), 505-510, 2004
一般社団法人 園芸学会
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詳細情報 詳細情報について
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- CRID
- 1390282681278146944
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- NII論文ID
- 110001815937
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- NII書誌ID
- AN00025257
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- COI
- 1:CAS:528:DC%2BD2cXhtVWhsLrL
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- ISSN
- 1880358X
- 00137626
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- NDL書誌ID
- 7151268
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- IRDB
- NDL
- Crossref
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可