長門ユズキチ果実フラベド抽出物のクロロフィル分解ペルオキシダーゼにより生じたフラボノイド・ラジカルによるクロロフィル分解

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タイトル別名
  • In Vitro Chlorophyll Degradation Involved in Flavonoid Radical Formed by Chlorophyll-degrading Peroxidase in Flavedo Extract of Citrus nagato-yuzukichi Fruit

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Effects of flavonoid radical formed by a chlorophyll (Chl)-degrading peroxidase in the flavedo extract of the green sour citrus, Nagato-yuzukichi (Citrus nagato-yuzukichi hort. ex Y. Tanaka) fruit on in vitro Chl degradation were determined. Adding hydrogen peroxide accelerated the Chl breakdown by the flavedo extract which included flavonoids and a Chl-degrading peroxidase. During Chl degradation, naringin, hesperidin, and neohesperidin were rapidly oxidized, but only naringin was involved in Chl degradation by Chl-degrading peroxidase. This enzymatic degradation in the flavedo extract was suppressed by the addition of scavengers of radical and superoxide anions. The superoxide formed by a xanthine-xanthine oxidase system and a superoxide dismutase, however, had little effect on Chl degradation. MnCl2, which is a superoxide anion scavenger, also inhibited the flavonoid oxidation. These findings indicate that the naringin radical formed by a Chl-degrading peroxidase in the flavedo could be related to Chl degradation.

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  • 園芸学会雑誌

    園芸学会雑誌 71 (2), 243-248, 2002

    一般社団法人 園芸学会

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