ピレスロイド抵抗性イエバエにおけるフェンバレレートの <i>in vitro</i> および <i>in vivo</i> 代謝

• 舩城 衛介

  Laboratory of Pesticide Toxicology. Faculty of Horticulture, Chiba University Agrochemical Research Department, Ube Research Laboratory, UBE Industries, Ltd.

• DAUTERMAN Walter Curl

  Department of Toxicology, North Carolina State University

• 本山 直樹

  Laboratory of Pesticide Toxicology. Faculty of Horticulture, Chiba University

書誌事項

タイトル別名

• <i>In vitro</i> and <i>in vivo</i> Metabolism of Fenvalerate in Pyrethroid Resistant Houseflies
• ピレスロイド抵抗性イエバエにおけるフェンバレレートのin vitroおよびin vivo代謝〔英文〕
• ピレスロイド テイコウセイ イエバエ ニ オケル フェンバレレート ノ in

抄録

In vitro and in vivo metabolism of phenoxyphenyl or chlorophenyl ring labeled ¹⁴C-fenvalerate was studied in pyrethroid resistant and susceptible strains of the housefly. Microsomes fortified with NADPH catalyzed the ester bond cleavage as well as ring hydroxylation at the 4′ position of the phenoxyphenyl group. Subcellular distribution, cofactor dependence, and inhibition with piperonyl butoxide and CO suggested that these reactions were catalyzed by the cytochrome P450-dependent monooxygenase system. Hydrolysis was responsible for a small portion of the ester bond cleavage. The largest interstrain difference was in the oxidative cleavage of the ester bond, which appeared to be due to a specific cytochrome P450 species present only in the pyrethroid resistant strain. Two organophosphate resistant strains with high oxidase activity did not show a significant difference in this activity compared to the susceptible strain. When equidoses of fenvalerate resulting in a LD₁₀ and a LD₉₀ were applied to the resistant and susceptible strains in vivo, the R/S ratio of excretion rate constant (hr^-1) for water-soluble metabolites was 3.8 and 56, respectively. This confirmed the importance of increased detoxification as an additional resistance mechanism as well as decreased nerve sensitivity as previously reported. Cuticular penetration of fenvalerate was not responsible for resistance in the strain studied.

収録刊行物

• 日本農薬学会誌（Ｊｏｕｒｎａｌ　ｏｆ　Ｐｅｓｔｉｃｉｄｅ　Ｓｃｉｅｎｃｅ）

  日本農薬学会誌（Ｊｏｕｒｎａｌ　ｏｆ　Ｐｅｓｔｉｃｉｄｅ　Ｓｃｉｅｎｃｅ） 19 (1), 43-52, 1994

  日本農薬学会

キーワード

• Health, Toxicology and Mutagenesis
• Insect Science

詳細情報

• CRID: 1390282680186927104
• NII論文ID: 110001828250
• NII書誌ID: AN00196227
• ISSN: 03851559
• ISSN: 13490923
• ISSN: 1348589X
• DOI: 10.1584/jpestics.19.43
• NDL書誌ID: 3869119
• Web Site: https://agriknowledge.affrc.go.jp/RN/2030511632
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I3869119
• Web Site: http://www.jstage.jst.go.jp/article/jpestics1975/19/1/19_1_43/_pdf
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10875896
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