Screening of medicinal plant extracts for estrogenic activity in combination with a glycosidase treatment Screening of medicinal plant extracts for estrogenic activity in combination with a glycosidase treatment

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Author(s)

    • AHN Eun-Mi
    • Institute of Natural Medicine, Faculty of Pharmaceutical Sciences
    • AKAO Teruaki
    • Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
    • NAKAMURA Norio
    • Institute of Natural Medicine, Faculty of Pharmaceutical Sciences
    • NISHIHARA Tsutomu
    • Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka University

Abstract

For the purpose of evaluating phytoestrogenic activity of medicinal plant extracts, a naringinase-pretreatment method was developed, monitoring with proliferation of MCF-7 human breast cancer cells and induction of β-galactosidase in a yeast two-hybrid assay system. 0f various medicinal plant extracts examined, the extracts of Alpinia katsumadai (seeds), Glycyrrhiza uralensis (roots) and Moghania philippinensis (roots) showed higher estrogenic activity by per-treatment with naringinase than the original extract themselves. The contents of liquiritigenin and isoliquiritigenin having potent estrogenic activity, appreciably increased after the naringinase treatment of the extract of G. uralensis. These findings suggested that orally administered crude drugs would increase their estrogenic activity, due to the hydrolysis of some glycosylated constituents by intestinal flora. 薬用植物エキスの植物エストロゲン活性を評価する目的で,naringinaseで前処理し,MCF-7ヒト乳癌細胞の増植反びyeast two-hybrid assay法によるβ-galactosidase活性の誘導を指標とする方法を開発した。検討したエキスの中でALpinia katsumadai, Glycyrrhiza uralensis, Moghania philippinensisはnaringinase処理することにより,元のエキスよりエストロゲン活性が強くなった。G. uralensisのエキスをnaringinase処理すると,エストロゲン活性のあるliquiritigeninとisoliquiritigeninの含有量は顕著に増加した。この結果は,生薬を経口投与すると場内細菌による配糖体の加水分解が起り,エストロゲン活性が増大することを示唆している。

For the purpose of evaluating phytoestrogenic activity of medicinal plant extracts, a naringinase-pretreatment method was developed, monitoring with proliferation of MCF-7 human breast cancer cells and induction of β-galactosidase in a yeast two-hybrid assay system. 0f various medicinal plant extracts examined, the extracts of Alpinia katsumadai (seeds), Glycyrrhiza uralensis (roots) and Moghania philippinensis (roots) showed higher estrogenic activity by per-treatment with naringinase than the original extract themselves. The contents of liquiritigenin and isoliquiritigenin having potent estrogenic activity, appreciably increased after the naringinase treatment of the extract of G. uralensis. These findings suggested that orally administered crude drugs would increase their estrogenic activity, due to the hydrolysis of some glycosylated constituents by intestinal flora.

Journal

  • J. Trad. Med.

    J. Trad. Med. 21(2), 81-86, 2004-04-20

    Medical and Pharmaceutical Society for WAKAN-YAKU

References:  24

Cited by:  3

Codes

  • NII Article ID (NAID)
    110001846624
  • NII NACSIS-CAT ID (NCID)
    AN10486121
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13406302
  • Data Source
    CJP  CJPref  NII-ELS  IR 
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