植物ホルモンのLC-MSによるマススペクトル

• 児島 清秀

  新潟大院自然研

• 高橋 みや子

  新潟大院自然研

• 大竹 憲邦

  新潟大濃

書誌事項

タイトル別名

• Mass spectrum of plant hormones by LC-MS

抄録

Mass spectrums were analyzed as drift voltage altered to determine the optimum condition in quantitative analysis of phytohormones (IAA, ABA and GA). In both IAA and ABA type, standard chemicals and marked ones showed almost same change of mass spectrum patterns. Optimum drift voltages for most intensity were 60 V in IAA and ABA type, 40 V in GA_3 and 80 V in GA_4. As to mass spectrums of all standards, it was confirmed the tendencies that as drift voltage increased, intensity of molecular ion peak decreased and fragment ion peak shifted to smaller m/z one. Drift voltages which existed molecular ion peak were 40 V in IAA type, 80 V in ABA type, 120 V in GA_3 and 140 V in GA_4. SIM method had 7 times higher sensitivity than Scan method, and was speculated to enable to determine the quantity in 100 pmol order.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 35 (0), 111-112, 2000

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390282679401500160
• NII論文ID: 110001853556
• DOI: 10.18978/jscrpanb.35.0_111
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可