25 トマトの単為結実性果実のアポプラスト中のIAA、GA_1及びCKs

• 児島 清秀

  新潟大農

• 田村 裕一

  新潟大農

書誌事項

タイトル別名

• IAA, GA_1 AND CKs IN APOPLAST OF PARTHENOCARPIC FRUIT OF TOMATO

抄録

Levels of indole-acetic acid (IAA), cytokinins (CKs) and gibberellic acid1 (GA_1) in seedless fruits of parthenocarpic tomato (cv. Rarkuna First) were analyzed. CKs included trans-zeatin (Z) and trans-zeatin riboside (ZR). Fruits were sampled at young stage (6 cm in a diameter) and mature green stage (8 cm in a diameter). Sampled fruits were sliced from equatorial region in 20% thickness of total fruit thickness, and sliced disks were separated into parts. The pericarps and partitions tissues were centrifuged for collection of apoplast solution (AP; sap out of a cell membrane). Thereafter the tissues were frozen, thawed and recentrifuged for collection of symplast solution (SP; sap within a cell). The locule tissues were homogenated and filtrated. At extraction, ^<13>C_6-IAA, ^2H_5-Z, ^2H_5-ZR, ^2H_2-GA_1 as internal standards were added. After purification, IAA, CKs and GA_1 were fractionated with HPLC and determined with LC-MS. IAA concentration was generally higher in 6cm-fruit than in 8cm-fruit. CKs concentrations were higher in partitions and locules tissues of 6cm-fruit (especially Z was 4.6 pmol/g fresh weight in SP of partition tissues and ZR was 55 in locule tissues), suggesting that partitions of fruits may be the site of Z synthesis. The concentrations of plant hormones in divided parts changed differently. Thus, precise determination of the hormone concentrations of the component tissues needs to assign specific roles in specific tissues.

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 37 (0), 59-60, 2002

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390001204424553472
• NII論文ID: 110001867860
• DOI: 10.18978/jscrpanb.37.0_59
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可