41. タバコ N 因子によるタバコモザイクウィルスの認識機構の解析

• 植田 浩一

  奈良先端科学技術大学院大学遺伝子教育研究センター

• 山口 夕

  奈良先端科学技術大学院大学遺伝子教育研究センター

• 佐野 浩

  奈良先端科学技術大学院大学遺伝子教育研究センター

書誌事項

タイトル別名

• Recognition of the tobacco mosaic virus by the tobacco N factor

抄録

Plant resist invading microbial pathogens by mounting rapid defense responses. For instance, tobacco plants carrying the N gene show a hypersensitive response (HR) against tobacco mosaic virus (TMV). This response can act only when the plant has a resistance gene and the pathogen has an avirulence gene. In the case of tobacco and TMV, N is a resistance gene product and the 50 kDa TMV helicase domain (p50) is an avirulence gene product. However, it is not yet known how the N factor recognizes p50. We investigated the interaction of the N protein and p50 by the yeast Two-hybrid system. When both the N protein and p50 are present, β-galactosidase activity is about 10 times higher as compared to the presence of only one of the two. Next, the specificity of the interaction was investigated by the growth test using a histidine synthase inhibitor (3-AT). Our results shown that, only when both the N factor and p50,but not one of the two alone, are present growth was possible. Several strains of TMV have been reported not to induce the HR in tobacco. One of these strains has an amino acid exchange in the hericase domain. A possible explanation of the no-HR phenotype would be a disturbed interaction with the N protein. For this reason we checked this mutated p50 in the yeast Two-hybrid assay. In contrast to the WT protein, the mutated p50 resulted in a very lowβ-galactosidase activity. Moreover, it did not grow in the 3-AT containing culture medium. The results suggest that p50 has combined with N factor directly

収録刊行物

• 植物化学調節学会 研究発表記録集

  植物化学調節学会 研究発表記録集 38 (0), 58-, 2003

  一般社団法人 植物化学調節学会

詳細情報

• CRID: 1390282679401575936
• NII論文ID: 110001899843
• DOI: 10.18978/jscrpanb.38.0_58
• ISSN: 21896313
• ISSN: 09191887
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可