Periodic Colony Formation by Bacterial Species<i>Bacillus subtilis</i>

• Wakita Jun-ichi

  Department of Physics, Chuo University, Kasuga, Bunkyo-ku, Tokyo 112-8551

• Shimada Hirotoshi

  Department of Physics, Chuo University, Kasuga, Bunkyo-ku, Tokyo 112-8551

• Itoh Hiroto

  Department of Physics, Chuo University, Kasuga, Bunkyo-ku, Tokyo 112-8551

• Matsuyama Tohey

  Department of Bacteriology, Niigata University School of Medicine, Niigata 951-8510

• Matsushita Mitsugu

  Department of Physics, Chuo University, Kasuga, Bunkyo-ku, Tokyo 112-8551

書誌事項

タイトル別名

• Periodic Colony Formation by Bacterial Species Bacillus subtilis.

抄録

We have investigated the periodic colony growth of bacterial species Bacillus subtilis. A colony grows cyclically with the interface repeating an advance (migration phase) and a rest (consolidation phase) alternately on a surface of semi-solid agar plate under appropriate environmental conditions, resulting in a concentric ring-like colony. It was found from macroscopic observations that the characteristic quantities for the periodic growth such as the migration time, the consolidation time and the terrace spacing do not depend so much on nutrient concentration C_n, but do on agar concentration C_a. The consolidation time was a weakly increasing function of C_a, while the migration time and the terrace spacing were, respectively, weakly and strongly decreasing function of C_a. Overall, the cycle (migration-plus-consolidation) time seems to be constant, and does not depend so much on both C_n and C_a. Microscopically, bacterial cells inside the growing front of a colony keep increasing their population during both migration and consolidation phases. It was also confirmed that their secreting surfactant called surfactin does not affect their periodic growth qualitatively, i.e., mutant cells which cannot secrete surfactin produce a concentric ring-like colony. All these results suggest that the diffusion of the nutrient and the surfactin are irrelevant to their periodic growth.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　ｔｈｅ　Ｐｈｙｓｉｃａｌ　Ｓｏｃｉｅｔｙ　ｏｆ　Ｊａｐａｎ

  Ｊｏｕｒｎａｌ　ｏｆ　ｔｈｅ　Ｐｈｙｓｉｃａｌ　Ｓｏｃｉｅｔｙ　ｏｆ　Ｊａｐａｎ 70 (3), 911-919, 2001

  一般社団法人 日本物理学会

キーワード

• pattern formation
• bacterial colony
• <I>Bacillus subtilis</I>
• periodic growth
• concentric ring-like pattern

詳細情報

• CRID: 1390001204180048256
• NII論文ID: 110001978977
• NII論文ID: 210000103595
• NII書誌ID: AA00704814
• DOI: 10.1143/jpsj.70.911
• BIBCODE: 2001JPSJ...70..911W
• ISSN: 13474073
• ISSN: 00319015
• ISSN: http://id.crossref.org/issn/00319015
• NDL書誌ID: 5732344
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5732344
• Web Site: http://journals.jps.jp/doi/pdf/10.1143/JPSJ.70.911
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可