Serum Consentration of 3-Hydroxy-5-Cholenoic Acid : Its Conjugated Sulfates and Glucuronides in Infants With Biliary Atresia

• Niinomi Noriji

  Department of Pepiatric Surgery, Anjo Kosei Hospital

• 堀沢 稔

  Department of Pepiatric Surgery, Anjo Kosei Hospital

• Ito Takahiro

  Department of Surgery, Branch Hospital, Nagoya University School of Medicine

• Ando Hisami

  Department of Surgery, Branch Hospital, Nagoya University School of Medicine

• Iio Kenji

  Department of Surgery, Branch Hospital, Nagoya University School of Medicine

• Okuyama Sumihiko

  Nagoya University College of Medical Technology

• Okumura Kazuyoshi

  Nagoya University College of Medical Technology

• Takagi Kenji

  Nagoya University College of Medical Technology

Bibliographic Information

Other Title

• 胆道閉鎖症における血清3β-Hydroxy-5-Cholenoic-Acidの測定意義と血清硫酸並びに血清グルクロン酸抱合型胆汁酸についての検討

Abstract

3-hydroxy-5-cholenoic acid which is one of monohydroxy bile acid with hepatotoxic effects, has been considered the product of an altered pathway of biosynthesis of bile acid in the liver. In the serum of cholestatic patients, it increases as a form of either sulfate-conjugated bile acid or glucuronide-conjugated bile acid. Bile acids in serum patients with biliary atresia and neonatal hepatitis were measured by high performance liquid chromatography (HPLC) combined with immobilized enzyme in colum form and new analytical method using sulfatase and β-glucuronidase. In preoperative biliary atresia group and neonatal hepatitis group, sulfate-conjugated bile acid was more dominant than glucuronide-conjugated bile acid. However, in postoperative biliary atresia group with persistent jaundice, consentration of glucuronide-conjugated bile acid was as much as that of sulfate-conjugated bile acid. Our studies suggested that glucuronide-conjugation might be related to development of UDP-glucuronyl-transferase activity and might play an important role in excreting abnormal bile acid into urine as well as sulfate-conjugation.

Journal

• Journal of the Japanese Society of Pediatric Surgeons

  Journal of the Japanese Society of Pediatric Surgeons 24 (4), 825-835, 1988

  The Japanese Society of Pediatric Surgeons

Keywords

• Bile acid
• Biliary atresia
• Neonatal hepatitis

Details

• CRID: 1390001204809717504
• NII Article ID: 110002099529
• DOI: 10.11164/jjsps.24.4_825
• ISSN: 21874247
• ISSN: 0288609X
• Web Site: https://search.jamas.or.jp/link/ui/1989063407
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed