Antiproliferative Effect of Tumor Necrosis Factor-α on Human Glioblastoma Cells Linked with Cell Cycle Arrest in G<SUB>1</SUB> Phase

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  • CHENG Kang
    Department of Neurosurgery, Hokkaido University School of Medicine
  • SAWAMURA Yutaka
    Department of Neurosurgery, Hokkaido University School of Medicine
  • SAKUMA Shirou
    Department of Neurosurgery, Hokkaido University School of Medicine
  • TADA Mitsuhiro
    Department of Neurosurgery, Hokkaido University School of Medicine
  • SUDO Masako
    Department of Neurosurgery, Hokkaido University School of Medicine
  • AIDA Toshimitsu
    Department of Neurosurgery, Hokkaido University School of Medicine
  • ABE Hiroshi
    Department of Neurosurgery, Hokkaido University School of Medicine

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The effects of tumor necrosis factor-α (TNF) on proliferation and cell cycle alterations in human malignant glioma cell lines, SF-188 and LN-382, were investigated by flow cytometry with the bromodeoxyuridine-propidium iodide dual staining technique. Low concentrations of TNF (1-100 U/ml) suppressed the growth of SF-188 assessed by cell count, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay, and thymidine incorporation assay, but not that of LN-382. After TNF treatment, the percentage of SF-188 cells in the G0/G1 phase increased, while the percentage of cells in the S phase decreased. LN-382 cells did not show any marked change in cell kinetics. TNF arrests certain human glioma cells in the G0/G1 phase resulting in reduction of deoxyribonucleic acid synthesis in the subsequent S phase, suppressing the proliferation pathway.

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