A New Automated Method to Analyze Urinary 8-hydroxydeoxyguanosine by a High-performance Liquid Chromatography-Electrochemical Detector System.

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  • KASAI HIROSHI
    Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health

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  • New Automated Method to Analyze Urinary 8 Hydroxydeoxyguanosine by a High performance Liquid Chromatography Electrochemical Detector System
  • A new automated method to analyze urinary 8-hydroxydeoxyguanosine by a high-performance liquid chromatography-electrochemical detection system

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Abstract

A new method was developed to analyze urinary 8-hydroxydeoxyguanosine (8-OH-dG) by high-performance liquid chromatography (HPLC) coupled to an electrochemical detector (ECD). This method is unique because (i) urine is first fractionated by anion exchange chromatography (polystyrene-type resin with quaternary ammonium group, sulfate form) before analysis by reverse phase chromatography; and (ii) the 8-OH-dG fraction in the first HPLC is precisely and automatically collected based on the added ribonucleoside 8-hydroxyguanosine marker peak, which elutes 4-5 min earlier. Up to 1,000 human urine samples can be continuously analyzed with high accuracy within a few months. This method will be useful for studies in radiotherapy, molecular epidemiology, risk assessment, and health promotion.<br>

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