Chlorophyll-deficient Mutants of Rice Demonstrated the Deletion of a DNA Fragment by Heavy-ion Irradiation
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- ABE TOMOKO
- Plant Functions Lab. RIKEN
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- MATSUYAMA TOMOKI
- Plant Functions Lab. RIKEN
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- SEKIDO SHIGEKO
- Plant Science Center, RIKEN
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- YAMAGUCHI ISAMU
- Plant Science Center, RIKEN
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- YOSHIDA SHIGEO
- Plant Functions Lab. RIKEN
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- KAMEYA TOSHIAKI
- Graduate School of Life Sciences, Tohoku University
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Heavy-ion irradiation is a new method of mutation breeding to produce new cultivars. We established the application of this method in rice plants to obtain mutants. Rice seeds were irradiated by C or Ne ions (135MeV/u) with a LET (linear energy transfer) of 22.7 or 64.2 keV/μm, respectively. Chlorophyll-deficient mutants (CDM) segregated in M2 progeny were albino, pale-green, yellow or striped-leave phenotypes. The highest rate of CDM with C-ion irradiation, 7.31%, was obtained at 40 Gy among the doses examined. Ne-ion irradiation gave the highest rate, 11.6%, at 20 Gy. We used the RLGS (Restriction Landmark Genomic Scanning) method to analyze DNA deletion in an albino mutant genome. Not I-landmark RLGS profiles detected about 2000 spots in rice. We found that one of the polymorphic spots was strongly linked to the albino phenotypic mutant derived from deleting of a DNA fragment, and demonstrated the high ability to detect of polymorphic regions by the RLGS method.<br>
収録刊行物
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- Journal of Radiation Research
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Journal of Radiation Research 43 (S), S157-S161, 2002
Journal of Radiation Research 編集委員会
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詳細情報 詳細情報について
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- CRID
- 1390001205217425408
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- NII論文ID
- 110002328974
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- NII書誌ID
- AA00705792
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- COI
- 1:CAS:528:DC%2BD3sXktlShtLc%3D
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- ISSN
- 13499157
- 04493060
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- NDL書誌ID
- 6523748
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- PubMed
- 12793751
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- NDL-Digital
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可