食物負荷試験用に製造した乾燥食品粉末のアレルゲン性のin vitroによる評価  [in Japanese] IN VITRO EVALUATION OF ALLERGENICITY OF DRIED FOOD POWDERS MANUFACTURED FOR FOOD PROVOCATION TEST  [in Japanese]

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Abstract

食物負荷試験(FPT)は食物アレルギーの重要な診断法の一つであるが, FPT用の標準抗原は開発されていない.本研究では,5種類の食物(牛乳,鶏卵,鶏肉,大豆および小麦)からスプレードライ法またはフリーズドライ法により粉末を製造し,FPT用抗原としての有用性をin vitroで調べた。SDS-PAGEでは,各粉末およびその原料の抽出液は同じまたは類似した泳動パターンを示した.食物アレルギー患児の血清を用いたELISAで分析したところ,各粉末抽出液の反応性と原料抽出液の反応性との間には良好な相関(r=0.853-0.978)がみられた。さらに,牛乳,鶏卵および大豆では,粉末抽出液および原料抽出液を固相化抗原として用いても阻害剤として用いても,ほぼ同じELISA阻害曲線が得られた.これらの結果から,各粉末は原料と同じアレルゲンを同レベルで含み,FPT用抗原として有用であると判断された.なお,粉末のアレルゲン性は-20℃または5℃貯蔵では18カ月以上安定であることも判明した.

Food provocation test (FPT) is one of important diagnostic methods for food allergy, but no standard antigens for FPT have yet been developed. In this study, dried powders were manufactured from five kinds of foods (cow's milk, hen egg, chicken, soybean and wheat) by spray-drying or freeze-drying and examined in vitro for their usefulness as antigens for FPT. In SDS-PAGE, the migration pattern of the extract from each powder was the same as or closely similar to that of the extract from its material. When analyzed by ELISA, a good correlation (r=0.853-0.978) in the reactivity with sera from food-allergic patients was observed between the extracts from each powder and its material. Moreover, in cow's milk, hen egg and soybean, almost the same ELISA inhibition curves were drawn, regardless of whether the extracts from each powder and its material were used as immobilized antigens or inhibitors. These results demonstrated that each powder contains the same allergens as its material at almost the same levels, being useful as an antigen for FPT. Favorably, the powders were found to be stored without significant changes in IgE reactivity at -20℃ or 5℃ for more than 18 months, although their storage at room temperature was suggested to be avoided.

Journal

  • Japanese Journal of Allergology

    Japanese Journal of Allergology 52(6), 522-529, 2003

    Japanese Society of Allergology

References:  6

Cited by:  4

Codes

  • NII Article ID (NAID)
    110002404114
  • NII NACSIS-CAT ID (NCID)
    AN00012583
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    0021-4884
  • NDL Article ID
    026061218
  • NDL Call No.
    Z19-32
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE 
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