AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE QUANTITATION OF THE MAJOR ALLERGEN FROM JAPANESE CEDAR (CRYPTOMERIA JAPONICA) POLLEN, Cry j I, USING MONOCLONAL ANTIBODIES
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- Watanabe Masanao
- Kowa Research Institute, Kowa Co., Ltd.
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- Tamura Masahiro
- Kowa Research Institute, Kowa Co., Ltd.
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- Nagoya Takao
- Kowa Research Institute, Kowa Co., Ltd.
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- Takahashi Yuichi
- The Yamagata Prefectural Institute of Public Health
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- Katagiri Susumu
- The Yamagata Prefectural Institute of Public Health
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- Oka Tetsuo
- Pharmacy, Tamano City Hospital
Bibliographic Information
- Other Title
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- スギ花粉抗原 (Cry j I) に対するモノクローナル抗体を用いたEnzyme-Linked Immunosorbent Assay (ELISA) によるCry j Iの測定
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Abstract
We have developed an enzyme-linked immunosorbent assay (ELISA) using two anti-Cry j I monoclonal antibodies (KW-S10 and KW-S131) for the quantitation of the major allergen from Japanese cedar (Cryptomeria Japonica) pollen, Cry j I. Polystyrene microplates coated with KW-S131 were incubated with pollen allergen extracts. Cry j I, which bound to the antibody, was detected with alkaline phosphatase-conjugated KW-S10 using chromogenic enzyme substrate. Cry j I could be measured in concentration of between 0.16 and 2.5 ng/ml by this assay. Intra- and inter-assay coefficients of variation were 1.1-3.5% and 0.9-4.6%, respectively. This assay was considered that it was specific for Cry j I of Japanese cedar pollen because it didn't react with allergens of hinoki (Chamaecypairs obtusa) pollen which have antigenicity in common to Japanese cedar pollen. This assay would be useful for the standardization of Japanese cedar pollen allergen extracts.
Journal
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- Japanese Journal of Allergology
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Japanese Journal of Allergology 41 (11), 1535-1539, 1992
Japanese Society of Allergology
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Keywords
Details 詳細情報について
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- CRID
- 1390282679953500288
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- NII Article ID
- 110002421147
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- ISSN
- 13477935
- 00214884
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- Text Lang
- ja
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed
