酸素プラズマによるエンドトキシン不活化法の開発

• 玉澤 かほる

  東北大学大学院 歯学研究科 口腔生物学講座 歯内歯周治療学分野

• 細渕 和成

  東京都立産業技術研究所・駒沢庁舎

書誌事項

タイトル別名

• Development of Endotoxin Inactivation by Oxygen Plasma

抄録

A new practical method for inactivating endotoxin (ET) was investigated using plasma ashing equipment for semiconductor (PACK-III, YAC Co., Ltd., Japan). Dry ET (5×10^5pg/glassware bottle, 100pg = 1EU) used as a sample in the present study was derived from Escherichia coli R3F653. With fixing output (150W), chamber temperature (60℃) and discharge time (10min), plasma processing was performed under 6 conditions. Three samples under each condition were processed, and the ET activity (ETA) was measured by the Limulus gelatin activity method. The results obtained were as follows. 1. The mean value of remained ETA processed with dry oxygen gas at 25Pa was smallest among 6 conditions, and was double digits decrease for unprocessed ETA. The value of processed ETA was significant difference among both gas types and gas pressures (repeated measured ANOVA, p<0.05). 2. The time required for the entire automatic plasma processing was 14minutes. 3. The chamber temperature during plasma processing was less than 70℃

収録刊行物

• 医科器械学

  医科器械学 74 (6), 285-291, 2004-06-01

  一般社団法人 日本医療機器学会

詳細情報

• CRID: 1390573980502089344
• NII論文ID: 110002514969
• NII書誌ID: AN00013123
• DOI: 10.4286/ikakikaigaku.74.6_285
• ISSN: 18814875
• ISSN: 0385440X
• Web Site: https://www.jstage.jst.go.jp/article/ikakikaigaku/74/6/74_KJ00000819547/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005087156
• 本文言語コード: ja
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可