大動脈の栄養血管の分布に関する新知見とそのLeakage現象について(第2報) : 浮腫性動脈反応に関する研究続報

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  • Demonstration of Vasa Vasorum in the Innermost Layers of Media of Dog's Aorta and their Vascular Leakage II. : Studies on the Edematous Arterial Reaction

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As shown in the previous report, we have succeeded in dernonstrating vasa vasorum in the innermost layers of media of the dog's aorta by an X-ray microscopic techniques. In the present study, electron microscopy was used to see the fine structure of vasavasorum and to see whether the histamine type of leakage (MAINO and PALADE, 1961) exist in the Vasa vasorum of the dog. Materials and Methods Six young and healthy male dogs, weighing 8.7-12.3 kg, were used. The dog was anaesthetized with 10ml of sodium pentobarbiturate. A dose of 2.5 ml/kg of carbon suspension (acting carbon Pelikan C11/143 1a) was injected into the cephalic vein of 3 dogs, and the aortic specimens were taken in situ 15 minutes after the injection. As a control, 2.5ml/kg of physiological saline was injected into the cephalic vein of another 3 dogs. Specimens of aorta were quickly removed from the surrounding tissue. The fixative was an I per cent aquous solution of osmium tetroxide with veronal buffer (pH= 7.4), which was brought to, isotonicity with 0.25 M of sucrose. This fixative was poured into the lumen of aorta, the specimen was cut into small blocks of about 1 to 2 mm x 3 to 4mm in size. The blocks were fixed for one hour under cold temperature in an ice-box and then dehydrated in serial changes of ethanol, and embedded in Epon 812 following the procedure of LUFT. The section was cut on a ultrotome-LKB stained with saturated aquenous solution of uranyl acetate. Electron microscopic photo-graphs were taken with a HITACHI-HU-11A microscope. Results 1. Fine filaments, about 50A in diameter, and small vesicles, 60mμ in diameter, were present in cytoplasm of almost all endothelial cells of the capillaries, arterioles and venules of vasa vasorurn. On the other hand myofilament-1ike structure are rare in the endothelial cells of aortic lumen. Some processes or microvilli were often seen projecting into the lumen of the vessel. Some of these microvilli or processes were actually small marginal folds and extended to the vessel lumen.

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