Identification of Chloroplast DNA Haplotypes of Abies firma and A. homolepis Using a Polymerase Chain Reaction with Species-Specific Primers :

• Isoda,Keiya

  Faculty of Agriculture, Kyushu University

• Shiraishi,Susumu

  Faculty of Agriculture, Kyushu University

抄録

DNA sequences of the chloroplast spacer region between the trnP and rrnW genes (234 bps) were determined for two Japanese fir species, Abies firma Sieb. et Zucc. and A. homolepis Sieb. et Zucc., using four individuals from each species. No intraspecific variation was found in either species, but interspecific sequence polymorphism was detected between the two species. The interspecific variation was three nucleotide changes, from the 148th to 150th nucleotide position. These three nucleotides were TAC in A. firma and GTA in A. homolepis, which were inverted. In order to identify the cpDNA haplotypes between the two species, this inversion polymorphism was utilized to develop a new marker. Species-specific primers were designed so that the 3 ' ends of the primers would anneal to the mutation site, in order for the two haplotypes to be easily identified by a polymerase chain reaction (PCR).

収録刊行物

• Journal of forest research

  Journal of forest research 4 (4), 291-294, 1999-11-16

  Japanese Forestry Society

キーワード

• Abies
• AS-PCR
• chloroplast DNA
• interspecific variation

詳細情報

• CRID: 1540854195338063104
• NII論文ID: 110002663918
• NII書誌ID: AA11074682
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10775161
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles