Enzymatic Synthesis of a Novel Trisaccharide, Glucosyl Lactoside.

• Shibuya Takashi

• Miwa Yoshikatsu

• Nakano Masayuki

• Yamauchi Takako

• Chaen Hiroto

• Sakai Shuzo

• Kurimoto Masashi

抄録

Cyclomaltodextrin glucanotransferase from Bacillus stearothermophilus produced a series of oligosaccharides by the transglycosylation reaction with cyclomaltohexaose as a glycosyl donor and D-lactose as its acceptor. The content of oligosaccharide A as the main transfer product was 22. 1% of the total sugar. The content was increased to 34. 1% by hydrolysis with glucoamylase. Oligosaccharide A was isolated by column chromatography and crystallized. By chemical and enzymatic analyses, oligosaccharide A was found to be a novel non-reducing trisaccharide ; O-β-_D-galactopyranosyl-(1→4)-O-β-_D-glucopyranosyl α-_D-glucopyranoside.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 57 (1), 56-60, 1993

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206469565952
• NII論文ID: 110002676140
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.57.56
• COI: 1:CAS:528:DyaK3sXkt1yntb4%3D
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 7763423
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.57.56
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可