Preparation of (1-13C)D-Glucose 6-Phosphate from (13C)Methanol and D-Ribose 5-Phosphate with Methylotrophic Enzymes.
Bibliographic Information
- Other Title
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- Preparation of [1-<sup>13</sup>C]<scp>D</scp>-Glucose 6-Phosphate from [<sup>13</sup>C]Methanol and<scp>D</scp>-Ribose 5-Phosphate with Methylotrophic Enzymes
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Abstract
[13C]Formaldehyde was selectively incorporated into the C-1 position of D-fructose 6-phosphate by condensation with D-ribulose 5-phosphate catalyzed by a partially purified enzyme system for formaldehyde fixation in Methylomonas aminofaciens 77a. Much of the [1-13C]D-fructose 6-phosphate produced in this reaction was converted to [1-13C]D-glucose 6-phosphate by the addition of glucose-6-phosphate isomerase. A fed-batch reaction with periodic additions of the substrates afforded 56.2g/liter D-glucose 6-phosphate and 26.8g/liter D-fructose 6-phosphate. When [13C]methanol was used as the C1-donor, the yield of [1-13C]D-glucose 6-phosphate was high when alcohol oxidase was added. The optimum conditions for sugar phosphate production in the fed-batch reaction gave 45.6g/liter [1-13C]D-glucose 6-phosphate and 16.4g/liter [1-13C]D-fructose 6-phosphate in 165min. The molar yield of the total sugar phosphates to methanol added was 95%. The addition of H2O2 and catalase to the reaction system supplied molecular oxygen for methanol oxidation to formaldehyde by alcohol oxidase.
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 57 (2), 308-312, 1993
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206470326528
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- NII Article ID
- 110002676207
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- NII Book ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed