Purification and Characterization of Two Lectins from the Sea Cucumber<i>Stichopus japonicus</i>
書誌事項
- タイトル別名
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- Purification and Characterization of Two Lectins from the Sea Cucumber Stichopus Japonicus.
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Two Ca2+-dependent lectins were purified from the sea cucumber Stichopus japonicus by affinity chromatography on lactosyl-Sepharose 4B and ion-exchange chromatography on Q-Sepharose. Their molecular masses were estimated to be 13 kDa (SJL-I) and 15 kDa (SJL-II) on SDS-PAGE. SJL-I agglutinated rabbit erythrocytes as well as human A, B, and O-type erythrocytes, but SJL-II agglutinated only rabbit erythrocytes. Hemagglutination by SJL-I was competitively inhibited by N-acetyl-D-galactosamine and galactose-containing carbohydrates. On the other hand, only lactose, melibiose, and raffinose gave weak inhibition of hemagglutination by SJL-II, suggesting that SJL-II may have high specificity for particular complex carbohydrate(s) on the surface of rabbit erythrocytes. SJL-II was activated at ten times lower Ca2+ concentration than SJL-I. Both lectins lost activity in acidic pH, while SJL-I appeared more stable down to pH 4.5.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 57 (10), 1736-1739, 1993
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681448318208
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- NII論文ID
- 110002676603
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- NII書誌ID
- AA10824164
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- ISSN
- 13476947
- 09168451
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- PubMed
- 7764270
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- PubMed
- CiNii Articles
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- 使用不可