Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells of<i>Pseudomonas fluorescens</i>TN5

• Nagasawa Toru

• Hurh Byungserk

• Yamane Tsuneo

書誌事項

タイトル別名

• Production of 6-Hydroxynicotinic Acid from Nicotinic Acid by Resting Cells of Pseudomonas fluorescens TN5.
• Production of 6-hydroxynicotinic acid by resting cells of Pseudomonas fluorescens TN5

抄録

Resting cells of Pseudomonas fluorescens TN5 catalyze the hydroxylation at the 6-position of nicotinic acid to produce 6-hydroxynicotinic acid. We optimized the reaction and culture conditions for the production of 6-hydroxynicotinic acid from nicotinic acid by using P. fluorescens TN5 resting cells. The addition of molybdenum and iron ions and nicotinic acid as an inducer to the culture medium significantly enhanced the formation of the hydroxylation enzyme. The supply of oxygen was important for the enhancement of hydroxylation activity. Under the optimum conditions, 98.7% of the added 1.4M nicotinic acid was converted to 6-hydroxynicotinic acid, and the highest yield was 191g of 6-hydroxynicotinic acid per liter of reaction mixture containing 7.43g dry weight of cells in 45 h at 35°C

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (4), 665-668, 1994

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681449060608
• NII論文ID: 110002676909
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.58.665
• COI: 1:CAS:528:DyaK2cXjtF2ju7o%3D
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.58.665
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可