Transglycosylation to Hesperidin by Cyclodextrin Glucanotransferase from an Alkalophilic<i>Bacillus</i>Species in Alkaline pH and Properties of Hesperidin Glycosides

• Kometani Takashi

• Terada Yoshinobu

• Nishimura Takahisa

• Takii Hiroshi

• Okada Shigetaka

書誌事項

タイトル別名

• Transglycosylation to Hesperidin by Cyclodextrin Glucanotransferase from an Alkalophilic Bacillus Species in Alkaline pH and Properties of Hesperidin Glycosides.
• Transglycosylation to hesperidin by cyclodextrin glucanotransferase from an alkaliphilic bacillus species in alkaline pH and properties of hesperidin glycosides

抄録

Cyclodextrin glucanotransferase [1, 4-α-D-glucan 4-α-D-(1, 4-glucano)-transferase, cyclizing ; CGTase, EC 2.4.1.19] from an alkalophilic Bacillus species produced hesperidin monoglucoside and a series of its oligoglucosides by the transglycosylation reaction with hesperidin as an acceptor and soluble starch as a donor. The formation of the glycosides was more effective at alkaline pHs than at neutral or acidic pHs, because of higher solubility of the acceptor. The structure of the purified monoglucoside was identified as 4^G-α-D-glucopyranosyl hesperidin by FAB-MS, α-, β-glucosidase and glucoamylase treatments, and methylation analysis. The solubility of both hesperidin mono and diglucoside in water was about 300 times higher than that of hesperidin, and they were found to have a stabilizing effect on the yellow pigment crocin, from fruits of Gardenia jasminoides, against ultraviolet radiation.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 58 (11), 1990-1994, 1994

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206473103360
• NII論文ID: 110002677262
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.58.1990
• COI: 1:CAS:528:DyaK2MXisVCrtLc%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.58.1990
• 本文言語コード: en
• データソース種別:
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