Degradation of 2-Ketoarginine by Guanidinobutyrase in Arginine Aminotransferase Pathway of<i>Brevibacterium helvolum</i>

• Yorifuji Takamitsu

• Kaneoke Mitsuoki

• Okazaki Takao

• Shimizu Eiichi

書誌事項

タイトル別名

• Degradation of 2-Ketoarginine by Guanidinobutyrase in Arginine Aminotransferase Pathway of Brevibacterium helvolum.

抄録

Guanidinobutyrase (EC 3.5.3.7) involved in the arginine oxygenase pathway of Brevibacterium helvolum IFO 12073 was found to catalyze also the hydrolysis of 2-ketoarginine (2-keto-5-guanidinovalerate) to 2-ketoornithine (2-keto-5-aminovalerate) and urea, the second step of the arginine aminotransferase pathway. No other enzyme that degraded 2-ketoarginine was found in cells grown on L-arginine. The enzyme hydrolyzed 2-ketoarginine with a relative rate of about 0.7% of that toward 4-guanidinobutyrate. The K_m for 2-ketoarginine was 33mM.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 59 (3), 512-513, 1995

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206474402688
• NII論文ID: 110002677484
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.59.512
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 7766193
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.59.512
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可