Cloning and Nucleotide Sequence of the Inulin Fructotransferase (DFA I-Producing) Gene of<i>Arthrobacter globiformis</i>S14-3

• Haraguchi Kazutomo

• Seki Koji

• Kishimoto Mamoru

• Nagata Tadahiro

• Kasumi Takafumi

• Kainuma Keiji

• Kobayashi Shoichi

書誌事項

タイトル別名

• Cloning and Nucleotide Sequence of the Inulin Fructotransferase (DFA I-Producing) Gene of Arthrobacter gloviformis S14-3.
• Cloning and nucleotide sequence of the inulin fructotransferase (DFA l-producing) gene of Arthrobacter globiformis S14-3
• Cloning and nucleotide sequence of the inulin fructotransferase (DFA 1 producing) gene of Arthrobacter globiformis S14-3

抄録

A gene encoding an inulin fructotransferase (DFA I-producing) [EC 2. 4. 1. 200] from Arthrobacter globformis S14-3 was cloned and the nucleotides sequenced, for the first time. The sequence indicated that the native enzyme protein is composed of 392 amino acid residues. The native enzyme is an extracellar enzyme produced in the culture supernatant of A. globformis S14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. The 1. 5-kb DNA fragment encoding the gene was found to produce the active enzyme in the culture supernatant of an E. coli clone, under the control of the lac promoter of pUC119.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 59 (10), 1809-1812, 1995

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206476276224
• NII論文ID: 110002677765
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.59.1809
• COI: 1:CAS:528:DyaK2MXptVKisL0%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• PubMed: 8534968
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.59.1809
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可