Application of the Upstream Region of a Bacillus Endoglucanase Gene to High-level Expression of Foreign Genes in Bacillus subtilis

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Author(s)

Abstract

A 0.4-kb ScaI-HpaI fragment, 199bp upstream of the structural gene for alkaline endoglucanase, from the alkalophilic Bacillus sp. KSM-64, was found to be essential for the extracellular production of the enzyme by recombinant Bacillus subtilis cells. We constructed a new vector, pHSP64 (5.5 kb), using pHY300PLK and part of the 5' region of the endoglucanase that contained a possible promoter region. Using recombinant B. subtilis cells that carried this vector, very high production of two endoglucanases and of chloramphenicol acetyltransferase was done.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 59(11), 2172-2175, 1995-11-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  20

Cited by:  15

Codes

  • NII Article ID (NAID)
    110002677862
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09168451
  • Data Source
    CJP  CJPref  NII-ELS  J-STAGE 
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