Purification and Characterization of a<i>β</i>-Glucosidase from<i>Polygonum tinctorium</i>, Which Catalyzes Preferentially the Hydrolysis of Indican

• MINAMI Yoshiko

• KANAFUJI Takeo

• MIURA Kazuo

書誌事項

タイトル別名

• Purification and Characterization of a .BETA.-Glucosidase from Polygonum tinctorium, Which Catalyzes Preferentially the Hydrolysis of Indican.
• Purification and characterization of a β-glucosidase from Polygonum tinctorium, which catalyzes preferentially the hydrolysis of indican

抄録

An enzyme, β-glucosidase, which hydrolyzes indican (indoxyl-β-D-glucoside) was isolated in pure form from a plant, Polygonum tinctorium, and characterized. The enzyme showed specifically a low K_m for indican (0.34mM). The enzyme reacted with some other β-glucosides having aromatic groups but not with α-glucoside or other glycosides like galactoside. The activity was inhibited by several metal cations such as Cu²⁺ and by typical inhibitors for β-glucosidase such as D-glucono-1, 5-lactone although these inhibitors required high concentrations.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (1), 147-149, 1996

  公益社団法人 日本農芸化学会

キーワード

• β-glucosidase
• glycoside
• indican
• indigo

詳細情報

• CRID: 1390001206473596928
• NII論文ID: 110002677954
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60.147
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60.147
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可