Purification and Characterization of a β-Glucosidase from Polygonum tinctorium, Which Catalyzes Preferentially the Hydrolysis of Indican

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著者

    • MINAMI Yoshiko
    • Okayama university of Science, Faculty of Science, Department of Biochemistry
    • KANAFUJI Takeo
    • Okayama university of Science, Faculty of Science, Department of Biochemistry
    • MIURA Kazuo
    • Okayama university of Science, Faculty of Science, Department of Biochemistry

抄録

An enzyme, β-glucosidase, which hydrolyzes indican (indoxyl-β-D-glucoside) was isolated in pure form from a plant, Polygonum tinctorium, and characterized. The enzyme showed specifically a low K_m for indican (0.34mM). The enzyme reacted with some other β-glucosides having aromatic groups but not with α-glucoside or other glycosides like galactoside. The activity was inhibited by several metal cations such as Cu^<2+> and by typical inhibitors for β-glucosidase such as D-glucono-1,5-lactone although these inhibitors required high concentrations.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 60(1), 147-149, 1996-01-23

    社団法人日本農芸化学会

参考文献:  25件中 1-25件 を表示

被引用文献:  7件中 1-7件 を表示

各種コード

  • NII論文ID(NAID)
    110002677954
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • データ提供元
    CJP書誌  CJP引用  NII-ELS  J-STAGE 
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