Isolation and Characterization of Tn5-Induced Mutants of Sphingomonas paucimobilis Defective in 2,5-Dichlorohydroquinone Degradation.

• NAGATA Yuji

• MIYAUCHI Keisuke

• SUH Seug-Kyo

• FUTAMURA Akiko

• TAKAGI Masamichi

Bibliographic Information

Other Title

• Isolation and Characterization of Tn<i>5</i>-Induced Mutants of<i>Sphingomonas paucimobilis</i>Defective in 2,5-Dichlorohydroquinone Degradation

Abstract

Sphingomonas paucimobilis UT26 uses γ-hexachlorocyclohexane (γ-HCH) as a sole source of carbon and energy. In UT26, γ-HCH is converted to 2, 5-dichlorohydroquinone (2, 5-DCHQ). To clone a gene that is involved in the degradation of 2, 5-DCHQ, a Tn5 mutation was introduced into UT26, and three mutants (UT102, UT103, and UT116) defective in 2, 5-DCHQ degradation were visually selected by spraying γ-HCH solution on the colonies. 2, 5-DCHQ and hydroquinone (HQ) degradation activities of these three mutants and UT26 were analyzed using resting cells. The three mutants had different phenotypes. It was also demonstrated that 2, 5-DCHQ and HQ degradation activities were inducibly expressed in UT26 in the presence of 2, 5-DCHQ.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 60 (4), 689-691, 1996

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• HCH
• chlorohydroquinone
• Sphingomonas
• biodegradation
• Tn5 mutagenesis

Details

• CRID: 1390282681451806336
• NII Article ID: 110002678101
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60.689
• COI: 1:CAS:528:DyaK28XislahsL4%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60.689
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed