Novel<i>β</i>-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from<i>Acremonium</i>sp. HI-25
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- Novel .BETA.-D-Galactofuranose-containing High-mannose Type Oligosaccharides in Ascorbate Oxidase from Acremonium sp. HI-25.
- Novel β-d-galactofuranose-containing high-mannose type oligosaccharides in ascorbate oxidase from Acremonium sp. HI-25
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Ascorbate oxidase from the fungus Acremonium sp. HI-25 is a copper-containing glycoprotein that catalyzes the oxidation of ascorbic acid to dehydroascorbic acid. Monosaccharide composition analysis showed that the enzyme contains exclusively N-linked oligosaccharide chains. Following liberation by hydrazinolysis/re-N-acetylation, and fractionation by HPLC on anion exchange, Amide-80 and/or octadecyl silica columns after derivatization with p-aminobenzoic ethyl ester, the structures of the twelve major neutral oligosaccharides were identified by FAB-MS, 400MHz 1H-NMR, methylation analysis, mild acid hydrolysis, and/or sequential exoglycosidase digestions. Acremonium sp. ascorbate oxidase was found to consist of high-mannose type oligosaccharides (76.3%) having 4 to 9 mannose residues and a series of novel D-galactofuranose-containing high-mannose type oligosaccharides (18.6%) with the following structure.[chemical formula]
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 60 (7), 1123-1130, 1996
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206474800896
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- NII論文ID
- 110002678207
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK28Xks1Gkt78%3D
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- ISSN
- 13476947
- 09168451
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- PubMed
- 8782408
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- 本文言語コード
- en
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- データソース種別
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- JaLC
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- PubMed
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- 使用不可