Structural Elucidation of N-Linked Sugar Chains of Storage Glycoproteins in Mature Pea (<i>Pisum sativum</i>) Seeds by Ion-spray Tandem Mass Spectrometry (IS-MS/MS)
書誌事項
- タイトル別名
-
- Structural Elucidation of N-Linked Sugar Chains of Storage Glycoproteins in Mature Pea (Pisum sativum) Seeds by Ion-spray Tandem Mass Spectrometry (IS-MS/MS).
- Structural elucidation of N-linked sugar chains of storage glycoproteins in mature pea (Pisum sativum) seeds by ion-spray tandem mass spectrometry
- Structural elucidation of N-liked sugar chains of storage glycoproteins in mature pea (Pisum sativum) seeds by ion-spray tandem mass spectrometry (IS-MS/MS)
この論文をさがす
抄録
The structures of N-linked sugar chains of the storage glycoproteins in mature pea seeds have been estimated. Nine pyridylaminated (PA-) N-linked sugar chains were derived and purified from the hydrazinolysate of the storage glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were first identified by two-dimensional PA-sugar chain mapping, considering the data of sugar composition analysis or sequential exoglycosidase digestions. The deduced structures were further analyzed by IS-MS/MS analysis. Every relevant fragment ion derived from all PA-sugar chains could be assigned on the basis of deduced structures. The estimated nine structures fell into two categories; the first was a typical oligomannose type (Man8-3GlcNAc2; 77.7%) which can be hydrolyzed by endo-β-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)], the second was a xylose-containing type (Man4-3Xyl1GlcNAc2, Man3Fuc1Xyl1GlcNAc2; 22.3%). Among these structures, Man8GlcNAc2 (19.7%), Man6GlcNAc2 (24.7%), and Man3Fuc1Xyl1GlcNAc2 (18.8%) were the dominant structures.
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 60 (11), 1841-1850, 1996
公益社団法人 日本農芸化学会
- Tweet
キーワード
詳細情報
-
- CRID
- 1390001206470490880
-
- NII論文ID
- 110002678382
-
- NII書誌ID
- AA10824164
-
- COI
- 1:CAS:528:DyaK28XntlKntLc%3D
-
- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
-
- PubMed
- 8987862
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可