Inhibition of Arachidonate Lipoxygenase Activities by 2-(3,4-Dihydroxyphenyl)ethanol, a Phenolic Compound from Olives.

• KOHYAMA Noriko

• NAGATA Tadahiro

• FUJIMOTO Shin-ichi

• SEKIYA Keizo

Bibliographic Information

Other Title

• Inhibition of Arachidonate Lipoxygenase

Abstract

The effects of olive fruit extract on arachidonic acid lipoxygenase activities were investigated using rat platelets and rat polymorphonuclear leukocytes (PMNL). Olive extract strongly inhibited both 12-lipoxygenase (12-LO) and 5-lipoxygenase (5-LO) activities. One of the compounds responsible for this inhibition was purified and identified as 2-(3, 4-dihydroxyphenyl)ethanol (DPE). DPE inhibited platelet 12-LO activity (IC₅₀, 4.2μM) and PMNL 5-LO activity (IC₅₀, 13μM) but not cyclooxygenase activity in cell-free conditions. It also inhibited 12-LO activity in intact platelets (IC₅₀, 50μM) and reduced leukotriene B₄ production in intact PMNL stimulated by A23187 (IC₅₀, 26μM). The inhibition by DPE of both lipoxygenase activities was stronger than that by oleuropein, caffeic acid, or 7 other related phenolic compounds, especially in intact cells. These results suggest that DPE is a potent specific inhibitor of lipokygenase activities.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 61 (2), 347-350, 1997

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• arachidonic acid
• lipoxygenase
• inhibitor
• olive
• phenolic compounds

Details

• CRID: 1390001206473542912
• NII Article ID: 110002678474
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.61.347
• COI: 1:CAS:528:DyaK2sXhs1Kjsb0%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL BIB ID: 4153773
• PubMed: 9058975
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4153773
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.347
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed