Potential<scp>d,l</scp>-Amino Acid Sequence Analysis of Peptides from the C-Terminus

• OHRUI Hiroshi

• ITOH Eigo

• NISHIDA Yoshihiro

• HORIE Hiroko

• MEGURO Hiroshi

書誌事項

タイトル別名

• Potential D,L-Amino Acid Sequence Analysis of Peptides from the C-Terminus.
• Potential D L Amino Acid Sequence Analy

抄録

A model tripeptide, Gly-L-Leu-L-Phe, was immobilized with activated aminomethyl polystyrene, and its C-terminal was reduced to an alcohol. This peptidyl alcohol was selectively hydrolyzed at the C-terminal amide bond to afford a polymer-supported dipeptide (Gly-L-Leu) and amino alcohol (Phe-OH). The amino alcohol, including it's absolute configuration, was determined by labelling with (+)-MNB-COOH, and the dipeptide was reused for a determination of its C-terminal amino acids. The D, L-amino acids of the tipeptide were sequentially determined from the C-terminus.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (2), 392-395, 1997

  公益社団法人 日本農芸化学会

キーワード

• C-terminal
• sequence analysis
• D, L-amino acid
• chiral amino alcohol

詳細情報

• CRID: 1390001206470233856
• NII論文ID: 110002678490
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.392
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4153785
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4153785
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.392
• 本文言語コード: en
• データソース種別:
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  • NDL
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  • CiNii Articles
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