Occurrence of Free <i>N</i>-Glycans in Pea (<i>Pisum sativum</i>, L) Seedlings^†

• KIMURA Yoshinobu

• TAKAGI Shigeaki

• SHIRAISHI Tomonori

書誌事項

タイトル別名

• Occurrence of Free N-Glycans in Pea (Pisum sativum. L) Seedlings.
• Occurrence of Free N-Glycans in Pea Pis

抄録

Free N-glycans have been found in pea seedlings. These free N-glycans were coupled with 2-aminopyridine and purified by gel filtration, Con A-Sepharose affinity chromatography, and size fractionation HPLC. These structures of pyridylaminated free N-glycans were analyzed by exomannosidase digestions and ionspray tandem mass spectrometry. The structural analyses showed that the several oligomannose-type sugar chains having one GlcNAc residue at the reducing-end side occur in the seedlings, suggesting the endo-β-N-acetylglucosaminidase PS [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 228-232 (1996)] should be involved in the release of oligomannose-type N-glycans from the storage glycoproteins [Y. Kimura et al., Biosci. Biotech. Biochem., 60, 1841-1850(1996)] during the germination of pea seeds.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (5), 924-926, 1997

  公益社団法人 日本農芸化学会

キーワード

• free N-glycans
• pea seedling
• endo-β-N-acetylglucosaminidase
• peptide
• N-glycanase
• ion-spray mass spectrometry

詳細情報

• CRID: 1390001206474298368
• NII論文ID: 110002678612
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.924
• COI: 1:CAS:528:DyaK2sXjsVyrsbg%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4220028
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4220028
• Web Site: https://www.tandfonline.com/doi/pdf/10.1271/bbb.61.924
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可