Purification and Characterization of a Glucose-tolerant β-Glucosidase from Aspergillus niger CCRC 31494

• YAN Tsong-Rong

• LIN Chun-Lieh

書誌事項

タイトル別名

• Purification and Characterization of a Glucose-tolerant .BETA.-Glucosidase from Aspergillus niger CCRC 31494.
• Purification and Characterization of a
• Purification and Characterization of a Glucose-tolerant<i>β</i>-Glucosidase from<i>Aspergillus niger</i>CCRC 31494
• Purification and characterization of a glucose-toleratnt β-glucosidase from Aspergillus niger CCRC 31494.
• Purification and characterization of a glucose-tolerant β-glucosidase from Aspergillus niger CCRC 3194
• Purification and characterization of glucose-tolerant β-glucosidase from Aspergillus niger CCRC 31494

抄録

An extracellular glucose-tolerant β-glucosidase was purified to homogeneity by alcohol fractionation and preparative isoelectric focusing from Aspergillus niger CCRC 31494. The enzyme was a dimeric protein with a subunit of 49, 000, and had its optimum activity at pH 5.0 and 55°C. The enzyme was completely inhibited by 5 mM Ag⁺. Thiol groups and serine residues were not essential for its activity. Low concentrations of alcohols (10%) except for methanol could activate the enzyme. It was very specific for para-nitrophenyl-β-D-glucoside (pNPG) and cellobiose. However, the enzyme also had some β-xylosidase activity, but showed no activity towards x-linked glycosidic substrates. The V_max of 124.4 U/mg and 21.6 U/mg were found for pNPG (K_m = 21.7 mM) and para-nitrophenyl-β-D-xyloside (pNPX) (K_m = 14.2 mM), respectively. The enzyme was tolerant to glucose inhibition with a K_i of 543 mM, while fructose, galactose, mannose, and xylose were not inhibitory.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (6), 965-970, 1997

  公益社団法人 日本農芸化学会

キーワード

• β-glucosidase
• Aspergillus niger
• glucose-tolerance
• purification
• characterization

詳細情報

• CRID: 1390001206475642368
• NII論文ID: 110002678620
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.965
• COI: 1:CAS:528:DyaK2sXkt1Cisro%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4240219
• PubMed: 9214755
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4240219
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.965
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可