Enzymatic Synthesis of Oligosaccharides Containing Galβ→4Gal Disaccharide at the Non-Reducing End Using β-Galactanase from Penicillium citrinum

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  • Enzymatic Synthesis of Oligosaccharides Containing Gal.BETA..RAR.4Gal Disaccharide at the Non-Reducing End Using .BETA.-Galactanase from Penicillium citrinum.
  • Enzymatic Synthesis of Oligosaccharides
  • Enzymatic Synthesis of Oligosaccharides Containing Gal<i>β</i>→4Gal Disaccharide at the Non-Reducing End Using<i>β</i>-Galactanase from<i>Penicillium citrinum</i>

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The transglycosylation reaction was done with a β-galactanase from Penicillium citrinum. The regioselectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and mono- or disaccharide derivatives containing β-galactosyl residue as acceptors. We also synthesized oligosaccharides containing Galβ1→4Gal sequence such as Galβ1→4Galβ1→4Glc, Galβ1→4Galβ1→3GlcNAc, Galβ1→4Galβ1→4GlcNAc, Galβ1→4Galβ1→6GlcNAc, and Galβ1→4Galβ1→3GalNAc for use in the total synthesis of complex sugar chains.

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