Purification and Characterization of Ferredoxin–Sulfite Reductase from Turnip (<i>Brassica rapa</i>) Leaves and Comparison of Properties with Ferredoxin–Sulfite Reductase from Turnip Roots

• TAKAHASHI Shunji

• YIP Wai-Cheung

• TAMURA Goro

書誌事項

タイトル別名

• Purification and Characterization of Ferredoxin-Sulfite Reductase from Turnip (Brassica rapa) Leaves and Comparison of Properties with Ferredoxin-Sulfite Reductase from Turnip Roots.
• Purification and Characterization of Fe

抄録

Ferredoxin-sulfite reductase (Fd-SiR) [hydrogen-sulfide: ferredoxin oxidoreductase, EC 1.8.7.1] from turnip leaves (SiR-L) has been purified to homogeneity and its enzymatic properties compared with that from turnip roots (SiR-R). Each enzyme had a molecular mass of 64.5±0.5 kDa by SDS-PAGE and an isoelectric point of 5.15±0.05. Although each had a pH optimum around 7.8 with the same effects of inhibitors, SiR-L had higher heat stability at 60°C than SiR-R. Moreover, SiR-R had a lower K_m and a higher specificity constant (k_cat/K_m) for turnip leaf ferredoxin than SiR-L. The N-terminal amino acid sequence of SiR-L was different from that of SiR-R. The results of amino acid analysis and peptide mapping suggested that SiR-L and SiR-R have different primary structures.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (9), 1486-1490, 1997

  公益社団法人 日本農芸化学会

キーワード

• ferredoxin-dependent enzyme
• non-photosynthetic tissue
• siroheme
• sulfate assimilation
• sulfite reductase

詳細情報

• CRID: 1390001206473082368
• NII論文ID: 110002678742
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.1486
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4307663
• PubMed: 9339549
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4307663
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.1486
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可