A Model System for Convenient Fluorescent Labeling of Sugar Chain in Taka-amylase A.

• KOBAYSHI Mikihiko

• SASAKI Yutaka

• CHIBA Yogo

書誌事項

タイトル別名

• Model System for Convenient Fluorescent

抄録

A convenient detection of sugar chains in Taka-amylase A (TAA) was done by using 40 μg of enzyme, where a decrease in the UV absorption of NaIO₄ during the periodate oxidation reaction was monitored. The periodate-oxidized sugar chain was labeled with a fluorescent reagent, N-1-ethylenediaminonaphthalene (EDAN), by incubation at pH 9.5 and 30°C for 1h. The excess EDAN was removed by either quenching with o-phthaladehyde or Bio-Gel P-2 gel adsorption. Among the peptide fragments preparbd from the EDAN-labeled TAA, a fluorescent peptide corresponding to the sugar chain was distinguished by the ODS column. These results suggest that periodate oxidation and subsequent fluorescent labeling were useful for the sensitive analysis of various glycoprotein samples.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (11), 1836-1839, 1997

  公益社団法人 日本農芸化学会

キーワード

• N- 1 -ethylenediaminonaphthalene
• fluorescent labeling
• periodate oxidation
• sugar chain
• Taka-amylase A

詳細情報

• CRID: 1390001206472755200
• NII論文ID: 110002678827
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.1836
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4357339
• PubMed: 9404062
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4357339
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.1836
• 本文言語コード: en
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