Transformation of the Edible Basidiomycete<i>Lentinus edodes</i>by Restriction Enzyme-Mediated Integration of Plasmid DNA
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- SATO Toshitsugu
- Iwate Biotechnology Research Center
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- YAEGASHI Kaori
- Iwate Biotechnology Research Center
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- ISHII Shizuko
- Iwate Biotechnology Research Center
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- HIRANO Tatsuya
- Iwate Biotechnology Research Center
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- KAJIWARA Susumu
- Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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- SHISHIDO Kazuo
- Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology
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- ENEI Hitoshi
- Iwate Biotechnology Research Center
書誌事項
- タイトル別名
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- Transformation of the Edible Basidiomycete Lentinus edodes by Restriction Enzyme-Mediated Integration of Plasmid DNA.
- Transformation of the edible basidiomycete Lentinula edodes by restriction enzyme-mediated integration of plasmid DNA
この論文をさがす
抄録
We have used the restriction enzyme-mediated DNA integration (REMI) method to establish a transformation system in Lentinus edodes using the recombinant plasmid pLC1-hph, which contains the L. edodes transcriptional signals and an Escherichia coli hygromycin B phosphotransferase gene. Protoplasts of L. edodes were treated by the PEG transformation mixture containing 50 units of SalI, which cleaves pLC1-hph at a single site, yielding about 15 transformants per 2.5 μg of DNA. The conventional PEG transformation without SalI, however, yielded only 1.5 transformants per 25 μg of DNA. The optimal amount of SalI for increased transformation was 50 units. In the case of transformation with SphI, which cleaves the plasmid at one site, the optimal amount of the enzyme was 2.5 units. Southern blot analysis of the SphI-derived transformants suggested that 50% of the plasmid integrations were REMI events.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (12), 2346-2350, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450114432
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- NII論文ID
- 110002679135
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXltV2isw%3D%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL書誌ID
- 973166
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- PubMed
- 9972260
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可