Purification and Characterization of Trehalose Phosphorylase from<i>Catellatospora ferruginea</i>
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- AISAKA Kazuo
- Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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- MASUDA Tomomi
- Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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- CHIKAMUNE Tadashi
- Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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- KAMITORI Kazuyo
- Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
書誌事項
- タイトル別名
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- Purification and Characterization of Trehalose Phosphorylase from Catellatospora ferruginea.
- Purification and Characterization of Tr
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抄録
Trehalose phosphorylase was purified from the cell extracts of Catellatospora ferruginea. The enzyme had an apparent molecular weight of 400,000 by gel filtration and 98,000 by SDS-PAGE, suggesting that the enzyme was a tetramer. The enzyme was specific for trehalose in phosphorolysis and specific for β-D-glucose 1-phosphate in synthesis. In addition to D-glucose, D-xylose and D-fucose were also possible sugar acceptors during synthesis. Phosphate ions were a key to the activity and stability of the enzyme, controlling the equilibrium of the reversible reaction and the heat stability of the enzyme. The enzyme was strongly inhibited by p-chloromercuribenzoate and pyridoxal phosphate. The enzyme was inactivated by heat or by storage frozen with ammonium chloride and lithium chloride.<br>
収録刊行物
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- Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ
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Ｂｉｏｓｃｉｅｎｃｅ， Ｂｉｏｔｅｃｈｎｏｌｏｇｙ， ａｎｄ Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (4), 782-787, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449692288
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- NII論文ID
- 110002679194
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXivFKlsbw%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL書誌ID
- 4473951
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- PubMed
- 9614710
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可