Molecular Cloning and Ethylene-Inducible Expression of<i>Chi</i>b1 Chitinase from Soybean (<i>Glycine max</i>(L.) Merr.)

• WATANABE Akira

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

• HAI NONG Van

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba Institute of Biotechnology, National Center for Natural Science and Technology

• ZHANG Deyu

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

• ARAHIRA Masaomi

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

• ASARE YEBOAH Nana

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

• UDAKA Kyoko

  Tokyo Kasei University

• FUKAZAWA Chikafusa

  Genetic Engineering Laboratory, National Food Research Institute, MAFF Tsukuba

書誌事項

タイトル別名

• Molecular Cloning and Ethylene-Inducible Expression of Chib1 Chitinase from Soybean (Glycine max (L.) Merr.).

抄録

  A soybean seed-specific PR-8 chitinase, named Chib2, has a markedly extended C-terminal segment compared to other plant Chib1 homologues of the PR-8 chitinase family known to date.<br>   To further characterize the molecular structure and the expression pattern of this chitinase family, we cloned two typical Chib1-similar cDNAs (Chib1-1 and Chib1-2) from soybeans by PCR-cloning techniques. The deduced primary sequence of Chib1-1 chitinase is composed of a signal peptide segment (26 amino acid residues) and a mature 273 amino acid sequence (calculated molecular mass 28,794, calculated pI 3.7). This Chib1-1 enzyme is more than 90% identical to Chib1-2 chitinase but is below 50% identical to Chib2 enzyme. Thus, we confirmed the occurrence of two distinct classes, Chib1 and Chib2 in the plant PR-8 chitinase family. The Chib1 genes, interrupted by one intron, were found to be up-regulated in response to ethylene in stems and leaves, but scarcely expressed in developing soybean seeds. Chib1 chitinases may be responsible for protecting the plant body from various pathogenic attacks.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 63 (2), 251-256, 1999

  公益社団法人 日本農芸化学会

キーワード

• acidic chitinases
• gene cloning
• ethylene-inducible expression
• PR-8 chitinase classification
• soybean

詳細情報

• CRID: 1390001206475725952
• NII論文ID: 110002679458
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.63.251
• COI: 1:CAS:528:DyaK1MXhs12gt7w%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4676492
• PubMed: 10192905
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4676492
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.63.251
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可