Chemical Structure and Molecular Weights of α-(1→3)-D-Glucan from Lentinus edodes

• ZHANG Pingyi

  Department of Chemistry, Wuhan University

• ZHANG Lina

  Department of Chemistry, Wuhan University

• CHENG Shuyao

  Department of Chemistry, Wuhan University

書誌事項

タイトル別名

• Chemical Structure and Molecular Weights of .ALPHA.-(1.RAR.3)-D-Glucan from Lentinus edodes.
• Chemical Structure and Molecular Weights of アルファ 1 3 D Glucan from Lentinus edodes
• Chemical Structure and Molecular Weights of α-(1→3)-<scp>D</scp>-Glucan from<i>Lentinus edodes</i>

抄録

  α-Glucan (L-FV-II) and β-glucan (L-FV-I) were isolated from fruiting bodies of Lentinus edodes by extraction with 5% NaOH/0.05% NaBH₄, then precipitation with 1 M acetic acid. The analysis results of Smith degradation, high-performance liquid chromatography (HPLC), infrared spectra (IR), ¹³C NMR spectra, and optical rotation indicated that L-FV-II is α-(1→3)-D-glucan with slight (1→6) branch linkages, and L-FV-I is β-(1→3)-D-glucan containing 10% protein, and with branches. The weight-average molecular weight, M_w, number-average molecular weight, M_n, radii of gyration, <s²>^1/2, and second virial coefficients, A₂, of L-FV-II in aqueous 0.5 M urea/0.5 M NaOH (obtained from diluting its solution in 1.0 M urea/1.0 M NaOH) and 0.25 M LiCl in dimethylsulfoxide (DMSO/0.25 M LiCl) were measured by light scattering, membrane osmometry, and size exclusion chromatography (SEC). The values of M_w and M_n for L-FV-II in 0.5 M urea/0.5 M NaOH are 24.1×10⁴ and 10.9×10⁴, respectively, similar to those in DMSO/0.25 M LiCl. It was proved that strong intermolecular hydrogen bonds exist in L-FV-II, resulting in its water-insolubility, but there are not any aggregates or multiple-helix structure for L-FV-II in DMSO/0.25 M LiCl. The chain of α-(1→3)-D-glucan is more extended in DMSO/0.25 M LiCl than that in the aqueous solution.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 63 (7), 1197-1202, 1999

  公益社団法人 日本農芸化学会

キーワード

• α-(1→3)-<small>D</small>-glucan
• <i>Lentinus edodes</i>
• ¹³C NMR
• molecular weight
• solution property

詳細情報

• CRID: 1390282681449730048
• NII論文ID: 110002679646
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.63.1197
• COI: 1:CAS:528:DyaK1MXlsFCntbY%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4820261
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4820261
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.63.1197
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可