Thermostabilization by Proline Substitution in an Alkaline,Liquefying α-Amylase from Bacillus sp. Strain KSM-1378
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- IGARASHI Kazuaki
- Tochigi Research Laboratories of Kao Corporation
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- OZAWA Tadahiro
- Tochigi Research Laboratories of Kao Corporation
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- IKAWA-KITAYAMA Kaori
- Tochigi Research Laboratories of Kao Corporation
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- HAYASHI Yasuhiro
- Tochigi Research Laboratories of Kao Corporation
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- ARAKI Hiroyuki
- Tochigi Research Laboratories of Kao Corporation
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- ENDO Keiji
- Tochigi Research Laboratories of Kao Corporation
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- HAGIHARA Hiroshi
- Tochigi Research Laboratories of Kao Corporation
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- OZAKI Katsuya
- Tochigi Research Laboratories of Kao Corporation
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- KAWAI Shuji
- Tochigi Research Laboratories of Kao Corporation
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- ITO Susumu
- Tochigi Research Laboratories of Kao Corporation
書誌事項
- タイトル別名
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- Thermostabilization by Proline Substitution in an lkaline, Liquefying .ALPHA.-Amylase from Bacillus sp. Strain KSM-1378.
- Thermostabilization by Proline Substitution in an Alkaline Liquefying アルファ Amylase from Bacillus sp. Strain KSM-1378
- Thermostabilization by Proline Substitution in an Alkaline, Liquefying α-Amylase from<i>Bacillus</i>sp. Strain KSM-1378
この論文をさがす
抄録
α-Amylase (LAMY) from alkaliphilic Bacillus sp. strain KSM-1378 is a novel semi-alkaline enzyme which has 5-fold higher specific activity than that of a Bacillus licheniformis enzyme. The Arg124 in LAMY was replaced with proline by site-directed mutagenesis to increase thermostability of the enzyme. The wild-type and engineered LAMYs were very similar with respect to specific activity, kinetic values, pH-activity curve, and degree of inhibition by chelating reagents. Thermostability and structure stiffness of LAMYs as measured by fluorescence were increased by the proline substitution. The change of Arg124 to proline is assumed to stabilize the loop region involving aminio acid residues from 122 to 134. This is the first report that thermostability of an α-amylase is improved by proline substitution.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 63 (9), 1535-1540, 1999
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473463424
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- NII論文ID
- 110002679709
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1MXmsVOktLo%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4863911
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- PubMed
- 10540739
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可