Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A_1aB_1bSubunit by Site-directed Mutagenesis

• TAKENAKA Yasuyuki

  Research Institute for Food Science, Kyoto University

• UTSUMI Shigeru

  Research Institute for Food Science, Kyoto University

• YOSHIKAWA Masaaki

  Research Institute for Food Science, Kyoto University

書誌事項

タイトル別名

• Introduction of Enterostatin (VPDPR) and a Related Sequence into Soybean Proglycinin A1aB1b Subunit by Site-directed Mutagenesis.

抄録

  Enterostatin (VPDPR), having anoretic and hypocholesterolemic activities, and its homologue LPYPR, a hypocholesterolemic peptide found in the glycinin A₅A₄B₃ subunit, were introduced into the corresponding site (TNGPQ) of the proglycinin A_1aB_1b subunit by site-directed mutagenesis. Modified proglycinins were expressed in E. coli and recovered from the insoluble fraction. VPDPR and LPYPR were released by the action of chymotrypsin and trypsin as expected. The overall yields of purified VPDPR and LPYPR were 40% and 62%, respectively.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (12), 2731-2733, 2000

  公益社団法人 日本農芸化学会

キーワード

• anoretic activity
• enterostatin
• glycinin
• hypocholesterolemic activity
• soybean

詳細情報

• CRID: 1390001206474284928
• NII論文ID: 110002679892
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.64.2731
• COI: 1:CAS:528:DC%2BD3MXmtVyhug%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 5628073
• PubMed: 11210149
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5628073
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.64.2731
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可