Role of Basic Residues of Humam Lactoferrin in the Interaction with B Lymphocytes
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- KAWASAKI Yoshihiro
- Technical Research Institute, Snow Brand Milk Products Co. Ltd.
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- SATO Kaoru
- Technical Research Institute, Snow Brand Milk Products Co. Ltd.
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- SHINMOTO Hiroshi
- Technical Research Institute, Snow Brand Milk Products Co. Ltd.
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- DOSAKO Shun’ichi
- Technical Research Institute, Snow Brand Milk Products Co. Ltd.
書誌事項
- タイトル別名
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- Role of Basic Residues of Human Lactoferrin in the Interaction with B lymphocytes.
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抄録
We have previously demonstrated that lactoferrin was incorporated into B lymphocytes and that a trypsin treatment for a short period reduced the number of lactoferrin molecules incorporated into B lymphocytes. An N-terminal sequence analysis revealed that the mild trypsin treatment had cleaved the three N-terminal amino acids, Gly1-Arg2-Arg3. Chemical conjugation of lost sequence analogue Gly-Arg-Arg-Gly with the mildly digested lactoferrin recovered the interaction with B lymphocytes, while conjugation of acetyl-Arg-Arg-Gly, a deamino analogue of Gly-Arg-Arg-Gly, did not recover the interaction. This shows that the N-terminal basic region containing N-terminal Gly played an important role in the interaction with B lymphocytes. Acylation of the amino groups of lactoferrin also significantly reduced the interaction with B lymphocytes, and an O-methylisourea treatment of the amino groups, which preserved the positive charge, hardly affected the interaction. These results suggest that both the N-terminal basic region and the basic characteristics of the whole molecule contributed to its interaction with B lymphocytes.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (2), 314-318, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681449948288
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- NII論文ID
- 110002679911
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXhvVCjtrc%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5296344
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- PubMed
- 10737187
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可