A Simple and Rapid Method for the Preparation of a Cell-free Extract with CCAAT-Binding Activity from Filamentous Fungi.

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  • KATO Masashi
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • HAYASHI Kojiro
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • KOBAYASHI Tetsuo
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University
  • TSUKAGOSHI Norihiro
    Department of Biological Mechanisms and Functions, Graduate School of Bioagricultural Sciences, Nagoya University

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  • Simple and Rapid Method for the Preparation of a Cell free Extract with CCAAT Binding Activity from Filamentous Fungi

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  A simple and rapid method for the preparation of a cell-free extract with the CCAAT-binding activity was established with Aspergillus nidulans as a model fungus. Proteins were extracted with 6 M guanidine hydrochloride directly from mycelia and renatured by dialysis. This method was found applicable to other filamentous fungi such as Aspergillus oryzae and Trichoderma viride.<br>

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