Binding of Barley and Wheat α-Thionins to Polysaccharides

• OITA Shigeru

  Shikoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries

• OHNISHI-KAMEYAMA Mayumi

  National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

• NAGATA Tadahiro

  National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries

書誌事項

タイトル別名

• Binding of Barley and Wheat .ALPHA.-Thionins to Polysaccharides.
• Binding of Barley and Wheat アルファ Thionins to Polysaccharides

抄録

  An antimicrobial peptide termed BCP-2 was purified from barley grain by chitin-affinity treatment and HPLC. The results of amino acid analysis and mass spectrometry of BCP-2 indicate that the peptide is very similar to barley α-thionin. BCP-2 and wheat α₁-thionin were also bound to β-glucan but not to starch. The binding of BCP-2 to laminarin (β-1,3-1,6-glucan) and laminarioligosaccharides was supported by fluorescence polarization data. This is the first report on the binding of α-thionins to polysaccharide containing chitin and β-1,3-glucan, which construct fungal cell walls.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (5), 958-964, 2000

  公益社団法人 日本農芸化学会

キーワード

• α-thionin
• chitin
• β-glucan
• binding peptide
• antifungal

詳細情報

• CRID: 1390282681450632832
• NII論文ID: 110002680026
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.64.958
• COI: 1:CAS:528:DC%2BD3cXjvFOqsbg%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 5404746
• PubMed: 10879464
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5404746
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.64.958
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可