Incorporation of the Whole Chromosomal DNA in Protoplast Lysates into Competent Cells of Bacillus subtilis.
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- AKAMATSU Takashi
- Department of Applied Microbial Technology, Sojo University
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- TAGUCHI Hisataka
- Department of Applied Microbial Technology, Sojo University
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Competent cells of Bacillus subtilis AC870 (purB, leuB, trpC, ald-1) were transformed to Ade+, Trp+, or Ade+ Trp+ with DNA in protoplast lysates of B. subtilis AC819 (hisH, tet-1, rpsL, smo-1). The contransfer ratio of purB to trpC was constant at 7-9% (Ade+ Trp+/Trp+) or 3% (Ade+ Trp+/Ade+) at protoplast concentrations of 2.7×103∼2.7×106 per ml. The whole chromosomal DNA must be certainly incorporated into competent cells from the following reasons; (1) purB is opposite to trpC on the chromosome, (2) 2.7×103 protoplasts per ml is about 100 times lower than 3.2×105 competent cells per ml, and (3) the cotransfer ratio is constant at all the concentrations. Similar results were obtained with the cotransfer ratio of purA to trpC. The transformation requires several Com proteins including ComK.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (4), 823-829, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206475852928
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- NII論文ID
- 110002680351
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXjsVagsLg%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5768847
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- PubMed
- 11388459
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
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- 使用不可