Efficient Synthesis of O-Linked Glycopeptide by a Transglycosylation Using Endo α-N-Acetylgalactosaminidase from Streptomyces sp

• AJISAKA Katsumi

  Nutrition Science Institute, Meiji Milk Products Co. Ltd.

• MIYASATO Mariko

  Nutrition Science Institute, Meiji Milk Products Co. Ltd.

• ISHII-KARAKASA Ikuko

  Department of Biochemistry, School of Medicine, Kitasato University

書誌事項

タイトル別名

• Efficient Synthesis of O-Linked Glycopeptide by a Transglycosylation Using Endo .ALPHA.-N-Acetylgalactosaminidase from Streptomyces sp.
• Efficient Synthesis of O Linked Glycopeptide by a Transglycosylation Using Endo アルファ N Acetylgalactosaminidase from Streptomyces sp

抄録

Galβ-(1→3)-GalNAc-linked hexapeptide was synthe-sized by a transglycosylation using Galβ-(1→3)-Gal-NAcβ-pNP as a donor and a serine-containing hexapeptide as an acceptor using endo GalNAc-ase from Streptomyces sp.. The Galβ-(1→3)-GalNAc residue was transferred to the hydroxyl group of the serine residue of the peptide. The total yield of the glycopeptide via this process was better than that of the chemoenzymatic method. This process was confirmed to be a versatile method for the synthesis of O-linked glycopeptides.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (5), 1240-1243, 2001

  公益社団法人 日本農芸化学会

キーワード

• glycopeptide synthesis
• mucin-type
• endo-α-N-acetylgalactosaminidase
• trans-glycosylation

詳細情報

• CRID: 1390282681452827776
• NII論文ID: 110002680425
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.65.1240
• COI: 1:CAS:528:DC%2BD3MXktFaitrY%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 5783003
• PubMed: 11440149
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5783003
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.65.1240
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可