帆立貝肝膵臓からのフコイタナーゼによるフコダインの酵素的加水分解

• KITAMURA Keiko

• MATSUO Masaru

• YASUI Tsuneo

書誌事項

タイトル別名

• Enzymic Degradation of Fucoidan by Fucoidanase from the Hepatopancreas of Patinopecten yessoensis.
• Enzymic Degradation of Fucoidan by Fucoidanase from the Hepatopancreas of<i>Patinopecten yessoensis</i>

抄録

A fucoidanase from the hepatopancreas of Patinopecten yessoensis was purified by ammonium sulfate precipitation, anion exchange chromatography, isoelectric focusing, and gel chromatography. The purified enzyme gave a single band on polyacrylamide gel electrophoresis. The fucoidanase was practically free from α-L-fucosidase and arylsulfatase activities. The molecular weight of the enzyme was estimated to be 85, 000 by gel filtration on TSKgel G3000SW and 84, 000 by SDS (sodium dodecyl sulfate) polyacrylamide gel electrophoresis. The enzyme hydrolyzed fucoidan to produce sulfated oligosaccharides as the reaction products.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (3), 490-494, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681448244224
• NII論文ID: 110002680562
• NII書誌ID: AN00128690
• DOI: 10.1271/bbb.56.490
• ISSN: 13476947
• ISSN: 09168451
• Web Site: https://tsc.repo.nii.ac.jp/records/2079
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.490
• 本文言語コード: en
• データソース種別:
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  • IRDB
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  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可