Cloning and Sequencing of the<i>xynA</i>Gene Encoding Xylanase A of<i>Aspergillus kawachii</i>

• ITO Kiyoshi

• IKEMASU Toru

• ISHIKAWA Takeaki

書誌事項

タイトル別名

• Cloning and Sequencing of the xynA Gene Encoding Xylanase A of Aspergillus kawachii.
• Cloning and sequencing of the xynA gene xylanase A of Aspergillus kawachii

抄録

We have cloned the xynA gene coding for xylanase A, a major commponent of the xylanase family, from Aspergillus kawachii. The cDNA was isolated from an A.kawachii cDNA library by immunoscreening using antibody raised against the purified xylanase A protein. Nucleotide sequence analysis of the cDNA showed a 981-bp open reading frame that encoded a protein of 327 amino acid residues. The signal peptide was composed of 25 amino acid residues and the N-terminus of the mature protein was pyroglutamic acid. The transformed yeast with a cloned cDNA prodcued xylanase. The genomic DNA was arranged as ten exons and nine introns.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (6), 906-912, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206471900544
• NII論文ID: 110002680638
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.906
• COI: 1:CAS:528:DyaK3sXktVersbk%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• PubMed: 1368254
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.906
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可