Synthesis of Functional Protein in Liposome

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Author(s)

Abstract

The liposome consisting of eggPC, cholesterol, and DSPE-PEG5000 with a molar ratio of 1.5:1:0.08 was used to entrap cell-free protein synthesis reaction mixture. The synthesis of a mutant green fluorescent protein in the liposome was confirmed by the fluorescence emitted from the liposome on flow cytometry analysis and fluorescence microscopy. The protein synthesized in the liposome is hence functional.

Journal

  • Journal of Bioscience and Bioengineering

    Journal of Bioscience and Bioengineering 92(6), 590-593, 2001-12-25

    The Society for Biotechnology, Japan

References:  22

Cited by:  5

Codes

  • NII Article ID (NAID)
    110002683967
  • NII NACSIS-CAT ID (NCID)
    AA11307678
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13891723
  • NDL Article ID
    6031103
  • NDL Source Classification
    ZP15(科学技術--化学・化学工業--醗酵・微生物工学)
  • NDL Call No.
    Z53-S65
  • Data Source
    CJP  CJPref  NDL  NII-ELS  J-STAGE  Crossref 
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